A re-routed bus service put in when another was withdrawn is now facing the axe.
Stagecoach has announced the 113 service between Preston and Wigan will cease in January.
The route was amended following the withdrawal of the 150/151 service between Preston and Bamber Bridge but the bus operator has blamed declining passenger numbers for its decision.
A petition set up against the ending of the service has seen more than 1,000 signatures.
Chorley Rural East councillor Kim Snape has set up the petition calling on Stagecoach CEO Martin Griffiths to act.
County councillor Snape said: “This will affect communities across South Ribble and the surrounding villages of Croston, Ulnes Walton, Eccleston along with many others. Some places would be left with little or no bus service provision.
“Public transport should be about providing a public service to residents not about profit.
“We are calling upon Stagecoach to think again and not go ahead with their proposals to withdraw this bus service.”
Read more: Woman tells of ‘humiliation’ on Stagecoach bus service in Preston
A statement from Stagecoach said: “From Monday 7th January, service 113 between Preston and Wigan will be withdrawn.
“The service has suffered from declining passenger numbers for a long of period of time, and the recent addition of alternative bus services in areas the 113 passes through, has increased this decline in passenger numbers. The 113, which receives no public funding, has now become unviable to operate.
“Stagecoach provided Lancashire Country Council with advance notice of this decision and will assist them wherever possible to provide alternative transport arrangements for communities affected.”
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Will you be affected by the change to the 113? Let us know in the comments below