Preston is to fly a rainbow flag at half mast on Tuesday in remembrance of those killed in Orlando.
Advertisement49 people died in the shooting early on Sunday morning at a gay club in America.
A further 53 people were injured in the attack by Omar Manteen, who was shot dead.
The attack is the deadliest mass shooting in recent US history and Preston is to show its solidarity with the United States.
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Cabinet member for environment councillor Robert Boswell said: “It’s such an appalling tragedy and our hearts go out to the victims and their families of this shocking event.
“It may have taken place far away but we are all still touched by the human emotion and out pouring of grief when so many people are killed and injured.
“We are flying the rainbow flag at half-mast as a mark of respect and in solidarity with the people of Orlando as well as the lesbian, gay, bi-sexual and trans-gender community who have been profoundly affected by this tragedy.”
The flag is to be flown from the Town Hall from 8am on Tuesday.