The Entertainer will team up with Derian House Children’s Hospital for their grand opening.
Olivia Rushton, who is the sibling ambassador for the hospice, will undertake a special trolley dash.
Her younger brother Joseph and sister Jess, who receives respite care at Derian House, will also be at the opening.
Derian House Children’s Hospice, said: “We’re delighted to be involved with the opening of this new store and very proud that Jess, Olivia and Joseph will be there to represent us.
“Olivia is such a fantastic sibling ambassador for Derian House and we are so touched because her and Joseph have already said they will donate the toys they manage to grab back to us for the children here.”
The store will open on Saturday, November in St George’s Centre.
Gary Grant, managing director and founder of The Entertainer, said: “After experiencing such solid growth across the UK, we are pleased to announce the opening of the Preston store.
“Preston and the centre itself continues to offer a strong retail position for our store as it attracts large numbers of family shoppers who are demanding more toy shopping choice on the high street and within shopping centres.
“We look forward to bringing joy and wonder to the children of Preston.”